Breaking Dormancy and Enhancing Germination of Avena sterilis L. and Amaranthus retroflexus L. Weeds by Gibberellic Acid and Potassium Nitrate to Keep Soil and Crops Healthy

Weeds represent one of the biggest obstacles facing agricultural production in the world because of the losses they cause in the productivity and quality of crops. For instance, in cereals, reduction in yields ranging from 50–99% based on the crop specie (El-Metwally and Saudy 2009; Saudy 2013; Saudy and Mubarak 2014; Saudy et al. 2021a, b). Furthermore, weeds reduced water use efficiency (El-Metwally et al. 2009; Saudy and El-Bagoury 2014; Saudy and El-Metwally 2022) and nutrient uptakes by crop plants (Saudy 2015; Saudy and Mubarak 2015; El-Metwally and Saudy 2021b). In several legume and oil crops distinctive deterioration in yield and quality were recorded owing to the presence of weeds (Aisa et al. 2019; Saudy 2009; El-Metwally and Saudy 2021a; Saudy et al. 2021c, 2022). On the other hand, dormancy is a strategy of weed survival and persistence threating continuously crop growers. Weed seed dormancy is one of the most pertinent adaptive properties that give various weeds a good chance of surviving and reproducing in a specific crop system. Seed dormancy is a feature found in many plant species whose adaptive importance is related to the ability to survive in unfavorable environments (Bewley et al. 2012). In croplands, the dormancy level of the weed seed bank in interaction with the predominating ecological conditions will determine the degree and timing of weed emergence (Batlla and Benech-Arnold 2010). However, Predicting the timing and extent of weed emergence in the field becomes difficult, when the seed is dormant (Benech-Arnold et al. 2000).

Dormant weeds had the potential to survive compared to the economic crops, especially under harsh conditions, and can germinate at some later time or in some other place (Tran and Cavanagh 1984). Persistence of the weed seed bank and timing of seedling emergence rely on dormancy (Walck et al. 2011; Batlla et al. 2020). Hence, weed seeds can remain in the soil for many years and germinate after experiencing favorable conditions for the survival of seedlings to maturation (Fenner 1985). Such a behavior drives to the accumulation of large seed amounts in the soil, shaping persistent banks which constitute the regenerative tactics developed by several weed species (Fenner 1985).

Avena fatua L. is a significant and widespread weed worldwide. It is a persistent weed with a combination of dormancy and seed longevity which allow viable seeds to infest agricultural soils for several years (Naylor 1983). Also, Amaranthus retroflexus is an important weed, which infests soil in several areas (Holm et al. 1997). Buried seeds of A. retroflexus can remain viable for at least 6–10 years (Costea et al. 2004).

Seeds dormancy is controlled by environmental factors that secure concurrence with the optimum growing season (Baskin and Baskin 1989). Breaking dormancy or stimulating germination is affected by several factors such as nitrate, temperature and light (Bouwmeester and Karssen 1993). Various treatments including plant growth regulators and chemicals have been applied to break seed dormancy in different species (Hartmann et al. 2010; Kaur et al. 2020).

It was recorded that gibberellic acid (GA₃) and potassium nitrate (KNO₃) treatments enhanced the germination of two Papaver species (Golmohammadzadeh et al. 2015), Cardaria draba (L.) Desv. (Rezvani and Zaefarian 2016), Myagrum perfoliatum L. (Honarmand et al. 2016), and Solanum americanum Mill. (Forte et al. 2019). Moreover, Rezvani and Zaefarian (2016) stated that GA₃, KNO₃ and pre-chilling activated the germination of hoary cress seeds. GA₃ had stimulation effects for seed germination (Lee et al. 2016; Urbanova and Leubner-Metzger 2018). Seeds soaked in 2% KNO₃ solution recorded approximately 63% increase in seed germination (Narmadha et al. 2020). However, despite the dangerous impacts of Avena sterilis L. and Amaranthus retroflexus L. in several field crops, their dormancy release was not clearly investigated.

Knowing the effective germination stimulants and their concentration as well as the optimal seedling emergence time has a practicable role in choosing the appropriate tillage time and system, the suitable application time of soil applied herbicides, and the proper crop sowing date. Therefore, the present study aimed to find the best chemical concentration of GA₃ and KNO₃ on dormancy breaking of Avena sterilis L. and Amaranthus retroflexus L. seeds. This could help in enhancing emergence potential of these weeds in field crops, which could be crucial in designing effective weed control programs.

The present work was carried out in Weed Research Central Laboratory, Agriculture Research Center (ARC), Giza, Egypt (30°01′ N, 31°12′ E). Four Laboratory experiments were conducted twice in sterilized glass Petri dishes.

Since weeds compete with crop plants for environmental factors, they should be eliminated. Due to weed competition, the availability of water and nutrients to the economic plants remarkably reduced (Saudy et al. 2020; El-Metwally et al. 2022a). Hence, quantitative and qualitative traits of the final product were significantly decreased (Saudy and Abd-Elmomen 2009; Saudy and El-Metwally 2009; El-Metwally et al. 2022b).

Certainly, emergency of weed plants from soil help the crop growers to distinguish the weed flora infested their fields. Thus, determination and planning tactics for weed control are becoming more easily. On the contrary, the presence and abundance of dormant weed seeds within soil represent a great challenge in weed management programs. Accordingly, obtaining germination stimulators is considered a remarkable tool for improving weed control methods, especially before crop planting to reduce weed infestation, hence plants grow in clean medium. The current work subjected to the dormancy as a critical issue of two important weeds, i.e. A. sterilis L. and A. retroflexus L. Findings exhibited that GA₃ and KNO₃ had motivative effect for A. sterilis L. weed germination and seedling growth. While, GA₃ alone was efficient for enhancing germination and seedling growth of A. retroflexus L. weed. Low and medium concentrations of GA₃ (150, 200 and 250 mg L⁻¹) and KNO₃ (250 and 500 mg L⁻¹) were more effective in this respect than the control or higher concentration (1000 mg L⁻¹). For promoting false jagged-chickweed seed germination rate, a KNO₃ concentration of 30 µmol and a GA₃ concentration of 144 µmol were the best treatments (Moeini et al. 2021).

In a wide range of plant species, due to its physiological role in induction of hydrolytic enzymes, GA₃ released the seed dormancy and promoted the germination (Rogis et al. 2004; Zhang et al. 2006) and can eliminate the natural chilling requirement of the dormant seeds (Gashi et al. 2012). Gibberellic acid could remove dormancy in Amaranthus retroflexus seeds and the dormancy release by GA₃ involved ethylene biosynthesis and action (Kępczyński et al. 2003). Sensitivity to GA₃ increases with the progress of dormancy release (Cadman et al. 2006; Finch-Savage and Leubner-Metzger 2006). The greatest germination percentage of Thlaspi arvense was obtained in seeds treated with 150 ppm GA₃ (Karimmojeni et al. 2014). A concentration range of GA₃ (288–1152 µmolar) increased germination of Erica andevalensis (Rossini Oliva et al. 2009). Applying pre-chilling with GA₃ provided the highest germination percentage in Helianthus tuberosus L. (Puttha et al. 2014).

Also, KNO₃ used for breaking seed dormancy and stimulating seed germination (Shim et al. 2008). In this respect, Chauhan et al. (2006) clarified that the germination rate of Sisymbrium orientale seeds increased in direct proportion to increasing concentrations of KNO₃, although only up to 0.02 M, after which it reduced. Stimulation and inhibition of seed germination at low and high concentrations of KNO₃ have previously been documented (Foley and Chao 2008; Wei et al. 2010). Potency of KNO₃ for breaking dormancy varied based on weed specie, since A. retroflexus L. was less affected than A. sterilis L. Potassium nitrate partly increased germination potential in Malcolmia africana seeds, which initially were less dormant than those of Thlaspi arvense and Descurainia Sophia (Karimmojeni et al. 2014). Various concentrations of KNO₃ promoted germination of false jagged-chickweed (Moeini et al. 2021) and other weed species such as Solanum rostratum and Cardaria draba (Rezvani and Zaefarian 2016; Wei et al. 2010).

Findings of the current research proved that the dormancy effectively broken by treating Avena sterilis L. seeds with gibberellic acid or potassium nitrate as well as Amaranthus retroflexus L. seeds with gibberellic acid. Generally, application of 150–250 mg L⁻¹ gibberellic acid and 250–750 mg L⁻¹ potassium nitrate regarded as the optimal dosages, since the highest increases in germination percentage and seedling growth traits were observed. Certainly, enhancing the germination of soil banked seeds of weeds especially prior crop planting will support the efficacy of weed control methods such as herbicides usage and false seed bed preparation. Thus, the crop competitiveness against weeds will improve, hence keeping soil and plant healthy.