Skip to main content
SpringerLink
Log in

Regeneration of doubled haploid plants by androgenesis of cucumber (Cucumis sativus L.)

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Six experiments (including pretreatment, embryonic callus induction media, preculture conditions, embryo induction media, embryo germination media, and genotypic effects) were conducted to develop an efficient cucumber (Cucumis sativus L., 2n = 2x = 14) anther culture protocol. Pretreatment and embryo induction were key factors for successful anther culture. Suitable temperature stress depended on the ecotype, i.e., cucumbers from cold areas responded well to cold shock whereas those from temperate areas responded well to heat treatment. The best medium for embryonic callus induction was MS medium supplemented with 4.44 μM BA, 2.26 μM 2, 4-D, 4.64 μM KIN, 3% sucrose and 0.8% agar. For embryo induction, MS medium supplemented with 0.54 μM NAA, 13.32 μM BA, 3% sucrose and 0.8% agar was optimal, and for embryo germination MS medium containing 2.22 μM BA, 6% sucrose and 1.2% agar was best. Using this protocol, we produced callus from 16 genotypes and regenerated plants from three of 20 evaluated. Three embryos per anther and 42 DH per 45 anthers (93% success) were obtained for cv. Ningjia No. 1, which was an improved result over a previous report. The origin of regenerants from microspores was determined by cytological, morphological and AFLP analyses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig 7

Similar content being viewed by others

Abbreviations

R0 :

Anther culture derived plants

R1 :

Selfed progeny of R0

DH:

Doubled haploid

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

6-benzyladenine

KIN:

Kinetin

TDZ:

Thidiazuron

NAA:

α-naphthaleneacetic acid

MS:

Murashige and Skoog (1962)

PGR:

Plant growth regulator

References

  • Amaury M, Arzate-Fernández, Tetsuya N, Hirotada Y, Takatoshi T (1997) Production of doubled-haploid plants from Lilium longiflorum Thunb. anther culture. Plant Sci 123:179–187

    Article  Google Scholar 

  • Chen JF, Qian CT (2002) Studies on chromosome preparations using plant tendril as source tissue. Acta Hort Sinica 29:378–380

    Google Scholar 

  • Ernst S, Scheibner K, Diettrich B, Luckner M (1990) Androgenetic cell cultures and plants from anthers of Digitalis lanata. J Plant Physiol 137:129–134

    CAS  Google Scholar 

  • Kumar HGA, Ravishankar BV, Murthy HN (2004) The influence of polyamines on androgenesis of Cucumis sativus L. Eur J Hort Sci 5:201–205

    Google Scholar 

  • Kumar HGA, Murthy HN (2004) Effect of sugars and amino acids on androgenesis of Cucumis sativus. Plant Cell Tiss Org Cult 78:201–208

    Article  CAS  Google Scholar 

  • Kumar HGA, Murthy HN, Paek KY (2003) Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L. Sci Hort 98:213–222

    Article  Google Scholar 

  • Lazarte JE, Sasser CC (1982) Asexual embryogenesis plantlet development in anther culture of Cucumis sativus L. HortScience 17:88

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murray HG, Thompson WF (1980) Rapid isolation of higher weight DNA. Nucleic Acids Res 8:4321

    Article  PubMed  CAS  Google Scholar 

  • Panaud O, Chen X, McCouch SD (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet 252:597–607

    PubMed  CAS  Google Scholar 

  • Rao PS, Suprassanna P (1996) Methods to double haploid chromosome numbers. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 1. Kluwer Academic Publ, Dordrecht pp 317–339

    Google Scholar 

  • SAS Institute Inc. (1999) SAS/STAT user’s guide, version 7-1. SAS Institute Inc, Cary, North Carolina

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van de lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Zhang X, Fowler SG, Cheng HM, Lou YG, Rhee SY, Stockinger EJ, Thomashow MF (2004) Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant Arabidopsis. Plant J 39:905–919

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the General Program 30470120 and 30671419 from the National Science Foundation of China; the 863 Program 2006AA10Z108, 2006BAD01A7-5-11, 2006AA100108 from the Ministry of Science and Technology of China. We are grateful to Dr Yong-Bing Zhang for his valuable suggestion of the manuscript. I wish to thank the Ms. Hong Geng and Yun-Feng Tan for providing intellectual support.

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Hui Song, Qun-Feng Lou, Xiang-Dong Luo, Joseph N. Wolukau, Wei-Ping Diao, Chun-Tao Qian & Jin-Feng Chen

Authors
  1. Hui Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qun-Feng Lou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiang-Dong Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Joseph N. Wolukau
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wei-Ping Diao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chun-Tao Qian
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jin-Feng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Feng Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Song, H., Lou, QF., Luo, XD. et al. Regeneration of doubled haploid plants by androgenesis of cucumber (Cucumis sativus L.). Plant Cell Tiss Organ Cult 90, 245–254 (2007). https://doi.org/10.1007/s11240-007-9263-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-007-9263-y

Keywords

Search

Navigation